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Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
DOI:

07:32 min

June 09, 2015

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Chapters

  • 00:05Title
  • 01:22Zygotic Embryo Development
  • 02:52Somatic Embryo Development In Vitro
  • 05:41Results: Representative Analyses of Somatic and Zygotic Embryo Development
  • 06:45Conclusion

Summary

Automatic Translation

The goal is to illustrate that the model legume Medicago truncatula can be readily utilized to investigate the regulation of early plant embryogenesis to complement the non-legume Arabidopsis model. Pod morphology is linked to zygotic embryogenesis stages and a protocol to collect embryos using tissue culture is also provided.

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