JoVE Journal
Biology
Biology
A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.
Chapters
Summary May 25th, 2015
Luminescent identification of functional elements in 3’ untranslated regions (3’UTRs) (3’LIFE) is a technique to identify functional regulation in 3’UTRs by miRNAs or other regulatory factors. This protocol utilizes high-throughput methodology such as 96-well transfection and luciferase assays to screen hundreds of putative interactions for functional repression.
