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Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Article DOI: 10.3791/52647 • 12:49 min
May 25th, 2015 •
1,2, 1,2, 2, 1,2
1School of Life Sciences, Arizona State University, 2Biodesign Institute, Arizona State University

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May 25th, 2015

Luminescent identification of functional elements in 3’ untranslated regions (3’UTRs) (3’LIFE) is a technique to identify functional regulation in 3’UTRs by miRNAs or other regulatory factors. This protocol utilizes high-throughput methodology such as 96-well transfection and luciferase assays to screen hundreds of putative interactions for functional repression.

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