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May 25, 2015
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Summary
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Luminescent identification of functional elements in 3’ untranslated regions (3’UTRs) (3’LIFE) is a technique to identify functional regulation in 3’UTRs by miRNAs or other regulatory factors. This protocol utilizes high-throughput methodology such as 96-well transfection and luciferase assays to screen hundreds of putative interactions for functional repression.
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Cite this Article
Wolter, J. M., Kotagama, K., Babb, C. S., Mangone, M. Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay. J. Vis. Exp. (99), e52647, doi:10.3791/52647 (2015).
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