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Detection of miRNA Targets in High-throughput Using the 3’LIFE Assay
Detection of miRNA Targets in High-throughput Using the 3’LIFE Assay
JoVE Journal
Biology
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JoVE Journal Biology
Detection of miRNA Targets in High-throughput Using the 3’LIFE Assay

Detection of miRNA Targets in High-throughput Using the 3’LIFE Assay

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12:49 min

May 25, 2015

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12:49 min
May 25, 2015

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Summary

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Luminescent identification of functional elements in 3’ untranslated regions (3’UTRs) (3’LIFE) is a technique to identify functional regulation in 3’UTRs by miRNAs or other regulatory factors. This protocol utilizes high-throughput methodology such as 96-well transfection and luciferase assays to screen hundreds of putative interactions for functional repression.

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