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Applying Stereotactic Injection Technique to Study Genetic Effects on Animal Behaviors
JoVE Journal
Behavior
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JoVE Journal Behavior
Applying Stereotactic Injection Technique to Study Genetic Effects on Animal Behaviors
DOI:

07:54 min

May 10, 2015

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Chapters

  • 00:05Title
  • 01:16Mouse Surgery and Microinjection
  • 04:50Open Field Test
  • 05:48Forced Swim Test
  • 06:45Results: The Effect of Virus Injection in Dentate Gyrus on Animal Behaviors
  • 07:33Conclusion

Summary

Automatic Translation

Stereotactic injection of lentiviruses expressing cDNAs or shRNAs can modulate gene expression in specific brain areas of mice. Here, we present a protocol to combine stereotactic injections with behavioral tasks, such as the Open Field Test (OFT) and the Forced Swim Test (FST).

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