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Immunology and Infection

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JoVE Journal Immunology and Infection
Utilisant la stratégie capside-Incorporation Antigen pour le développement d'approches adénovirus sérotype 5 Vectored vaccins
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Utilisant la stratégie capside-Incorporation Antigen pour le développement d'approches adénovirus sérotype 5 Vectored vaccins

Article DOI: 10.3791/52655-v • 13:36 min • May 6th, 2015
May 6th, 2015 •
1, 1, 1, 1,2
1Department of Medicine, University of Alabama at Birmingham, 2Center for AIDS Research, University of Alabama at Birmingham

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Ici, nous présentons un protocole pour générer un type bivalent adénovirus 5 (Ad5) vecteur preuve-de-principe Ad5 / H5-HVR1-Kwas-HVR5-His 6 en utilisant la stratégie Antigène capside-Incorporation. Ce vecteur a été démontrée à exposer remise en forme qualitative, la capacité d'échapper sérums Ad5 positif in vitro, et l'antigénicité ainsi que l'immunogénicité des antigènes incorporés.

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Immunologie Numéro 99 la stratégie Antigène capside-Incorporation méthode de transgène adénovirus (Ad) vaccins protéines de capside double modification l'immunité pré-existante (PEI)
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