Journal
/
Immunology and Infection
/
Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

DOI:
10.3791/52655-v

•

13:36 min

•

May 06, 2015

•

, , ,
1Department of Medicine,University of Alabama at Birmingham, 2Center for AIDS Research,University of Alabama at Birmingham

Chapters

  • 00:05Title
  • 02:31Construction of Modified Plasmid pAd5/H5-HVR1-KWAS-HVR5-His6
  • 05:09Preparation of Modified Ad5 Viral Vector Ad5/H5-HVR1-KWAS-HRV5-His6
  • 09:50Validation of the Rescued Viral Vector
  • 11:22Results: Evaluation of the Divalent Ad5 Vector Ad5/H5-HVR1-KWAS-HVR5-His6
  • 13:02Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to generate a proof-of-principle divalent adenovirus type 5 (Ad5) vector Ad5/H5-HVR1-KWAS-HVR5-His6 by utilizing the Antigen Capsid-Incorporation strategy. This vector was demonstrated to exhibit qualitative fitness, the capability to escape Ad5-positive sera in vitro, and the antigenicity as well as immunogenicity to the incorporated antigens.

Tags

Keywords: Adenovirus Serotype 5Ad5Antigen Capsid-incorporation StrategyVaccine DevelopmentHIV EpitopeHis6 TagHexonHypervariable RegionNeutralization AssayHumoral ImmunityRare-serotype Adenovirus

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52655?article_type=v

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
check_url/52655?article_type=v

Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems
check_url/52655?article_type=v

Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells
check_url/52655?article_type=v

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
check_url/52655?article_type=v

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer’s Patches Using Adoptive Transfer of Hematopoietic Progenitors
check_url/52655?article_type=v

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
check_url/52655?article_type=v

Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology
check_url/52655?article_type=v

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
check_url/52655?article_type=v

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis
check_url/52655?article_type=v

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity

Read Article