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Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis
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JoVE Journal Medicine
Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis

Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis

DOI:
10.3791/52665-v

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10:04 min

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May 01, 2015

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, , ,
1Department of Internal Medicine V,Innsbruck Medical University, 2Oncotyrol GmbH, 3Tyrolean Cancer Research Institute, 4Division of Vascular Biology, Department of Medical Biochemistry and Biophysics,Karolinska Institute

Chapters

  • 00:05Title
  • 02:09Cell Culture and Lentiviral Transfection
  • 03:203D-Multiple Myeloma Spheroid Model
  • 04:473D Multiple Myeloma Xenograft Model in the CAM
  • 06:17Quantification of eGFP Protein by ELISA
  • 07:03Immunohistochemical Analysis of Blood Vessels and Invading Tumor Cells
  • 08:05Results: Bortezomib Treatment of Multiple Meyloma Xenografts
  • 09:17Conclusion

Summary

Automatic Translation

Automatic Translation

Human multiple myeloma (MM) cells require the supportive microenvironment of mesenchymal cells and extracellular matrix components for survival and proliferation. We established an in vivo chicken embryo model with engrafted human myeloma and mesenchymal cells to study effects of cancer drugs on tumor growth, invasion and angiogenesis.

Tags

Keywords: Multiple MyelomaXenograft ModelChicken Chorioallantoic Membrane3D CultureAngiogenesisTumor GrowthInvasionGFP ReporterMesenchymal CellsCollagenRPMI-8226OPM-2Therapeutic Screening

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