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Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling
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Immunology and Infection
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JoVE Journal Immunology and Infection
Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling

Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling

DOI:
10.3791/52687-v

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09:43 min

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April 16, 2015

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, , , ,
1LD MacLean Surgical Research Laboratories, Department of Surgery,McGill University

Chapters

  • 00:05Title
  • 01:14Neutrophil Isolation from Whole Blood
  • 03:38Neutrophil Extracellular Trap (NET) Generation and Isolation
  • 05:24NET-cancer Cell Static Adhesion Assay
  • 08:18Results: Simple Generation and Isolation of NETs from Human Whole Blood
  • 09:12Conclusion

Summary

Automatic Translation

Automatic Translation

In the following protocol, we describe a very simple way to isolate Neutrophil Extracellular traps (NETs) from human whole blood using readily available reagents. We then demonstrate how the isolated NETs can be used in an in vitro adhesion assay with cancer cells.

Tags

Keywords: Neutrophil Extracellular Traps (NETs)NET IsolationNET HandlingCell-free NET IsolationNET-based AssaysNET Adhesion AssayA549 Lung Cancer CellsPMA StimulationDensity Gradient SeparationDNase1

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