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JoVE Journal
Immunology and Infection

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Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling
 

Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling

Article DOI: 10.3791/52687
April 16th, 2015

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Summary April 16th, 2015

In the following protocol, we describe a very simple way to isolate Neutrophil Extracellular traps (NETs) from human whole blood using readily available reagents. We then demonstrate how the isolated NETs can be used in an in vitro adhesion assay with cancer cells.

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