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An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples
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JoVE Journal Developmental Biology
An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

DOI:
10.3791/52694-v

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08:58 min

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April 12, 2015

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, , , , , ,
1Swiss Paraplegic Research, 2Orthopaedics and Spinal Surgery,Swiss Paraplegic Centre, 3Department of Neurosurgery,Lucerne Cantonal Hospital (LUKS), 4Section of Small Animal Surgery/Neurology,Vetsuisse Faculty, University of Zurich

Chapters

  • 00:05Title
  • 01:16Preparation of Urokinase and Enzymatic Digestion of Clot
  • 03:13Seeding for Expansion of Cell Culture and CFU Plates
  • 04:13Giemsa Stain for CFU Assay
  • 05:00Canine MSC Differentiation
  • 06:52Results: Mesenchymal Stem Cell Rescue from Clotted Bone Marrow
  • 08:17Conclusion

Summary

Automatic Translation

Automatic Translation

Mesenchymal stem cells are usually obtained from bone marrow and require expansion culture. When samples clot before processing, a protocol using the (enzymatic) thrombolytic drug urokinase can be applied to degrade the clot. Thus, cells are released and available for expansion culture. This protocol provides a rapid and inexpensive alternative to resampling.

Tags

Keywords: Mesenchymal Stem CellsBone MarrowUrokinaseClot DigestionCell ExpansionAutologous Stem Cell TherapyHematopoietic SystemClot RemovalEnzymatic DigestionCell QualityChondrogenicOsteogenicAdipogenic Differentiation

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