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Developmental Biology

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JoVE Journal Developmental Biology
An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples
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An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

Article DOI: 10.3791/52694
April 12th, 2015 •
*1, *1, 1, 2, 3, 4, 1
1Swiss Paraplegic Research, 2Orthopaedics and Spinal Surgery, Swiss Paraplegic Centre, 3Department of Neurosurgery, Lucerne Cantonal Hospital (LUKS), 4Section of Small Animal Surgery/Neurology, Vetsuisse Faculty, University of Zurich
* These authors contributed equally

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Summary
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April 12th, 2015

Mesenchymal stem cells are usually obtained from bone marrow and require expansion culture. When samples clot before processing, a protocol using the (enzymatic) thrombolytic drug urokinase can be applied to degrade the clot. Thus, cells are released and available for expansion culture. This protocol provides a rapid and inexpensive alternative to resampling.

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