Immunostaining to Visualize Murine Enteric Nervous System Development

Amanda J. Barlow-Anacker; Christopher S. Erickson; Miles L. Epstein; Ankush Gosain

doi:10.3791/52716

Immunostaining to Visualize Murine Enteric Nervous System Development

JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Developmental Biology

Immunostaining to Visualize Murine Enteric Nervous System Development

Full Text

11,992 Views

07:54 min

April 29, 2015

DOI: 10.3791/52716-v

Amanda J. Barlow-Anacker¹, Christopher S. Erickson¹, Miles L. Epstein², Ankush Gosain^1,2,3

¹Department of Surgery,University of Wisconsin School of Medicine and Public Health, ²Department of Neurosciences,University of Wisconsin School of Medicine and Public Health, ³Department of Pediatrics,University of Wisconsin School of Medicine and Public Health

The enteric nervous system is formed by neural crest cells that proliferate, migrate and colonize the gut. Neural crest cells differentiate into neurons with markers specific for their neurotransmitter phenotype. This protocol describes a technique for dissecting, fixing and immunostaining of the murine embryonic gastrointestinal tract to visualize enteric nervous system neurotransmitter expression.

Explore More Videos

Enteric Nervous System Neural Crest Cells Cholinergic Neurons Choline Acetyltransferase (ChAT)Immunostaining Embryonic Development Gastrointestinal Tract Neurotransmitter Expression