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Immunostaining to Visualize Murine Enteric Nervous System Development
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Developmental Biology
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JoVE Journal Developmental Biology
Immunostaining to Visualize Murine Enteric Nervous System Development

Immunostaining to Visualize Murine Enteric Nervous System Development

DOI:
10.3791/52716-v

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07:54 min

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April 29, 2015

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, , ,
1Department of Surgery,University of Wisconsin School of Medicine and Public Health, 2Department of Neurosciences,University of Wisconsin School of Medicine and Public Health, 3Department of Pediatrics,University of Wisconsin School of Medicine and Public Health

Chapters

  • 00:05Title
  • 01:41Embryo and Gut Dissection
  • 03:38Fixation of GI Tracts
  • 04:34Immunostaining Protocol
  • 06:11Results: Representative Images of Cholinergic Neurons in the Distal Small Intestine and Colon
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

The enteric nervous system is formed by neural crest cells that proliferate, migrate and colonize the gut. Neural crest cells differentiate into neurons with markers specific for their neurotransmitter phenotype. This protocol describes a technique for dissecting, fixing and immunostaining of the murine embryonic gastrointestinal tract to visualize enteric nervous system neurotransmitter expression.

Tags

Keywords: Enteric Nervous SystemNeural Crest CellsCholinergic NeuronsCholine Acetyltransferase (ChAT)ImmunostainingEmbryonic DevelopmentGastrointestinal TractNeurotransmitter Expression

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