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Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration
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Chapters
- 00:05Title
- 01:22Harvesting Cells
- 02:32Preparing the Collagen Solutions
- 03:52Formation of Concentric Gel Cultures
- 06:54Live-Cell Imaging of Concentric Gel Cultures
- 08:16Tracking Cell Movement
- 09:38Results: Cell Movement Analysis
- 11:03Conclusion
The mechanical properties and microstructure of the extracellular matrix strongly affect 3D migration of cells. An in vitro method to study the spatiotemporal cell migration behavior in biophysically variable environments, at both population and individual cell levels, is described.
