Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

Nicholas Agung Kurniawan; Parthiv Kant Chaudhuri; Chwee Teck Lim

doi:10.3791/52735

Journal

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Bioengineering

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Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

8,469 Views

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11:43 min

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April 03, 2015

DOI:

10.3791/52735-v

DOI:

10.3791/52735-v

•

11:43 min

April 03, 2015

•

2 Views

Nicholas Agung Kurniawan¹, Parthiv Kant Chaudhuri², Chwee Teck Lim^2,3

¹Systems Biophysics Department,FOM Institute AMOLF, ²Mechanobiology Institute,National University of Singapore, ³Department of Biomedical Engineering,National University of Singapore

Summary

The mechanical properties and microstructure of the extracellular matrix strongly affect 3D migration of cells. An in vitro method to study the spatiotemporal cell migration behavior in biophysically variable environments, at both population and individual cell levels, is described.