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마우스 나이브 CD4<sup> +</sup> T 세포의 분리 및<em> 시험관</em> T 세포 하위 집합으로 차별화
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
DOI:

07:12 min

April 16, 2015

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Chapters

  • 00:05Title
  • 01:09Mouse Lymph Node and Spleen Isolation
  • 02:11Tissue Processing and CD4+ Enrichment
  • 04:22Naïve CD4+ T Cell Sorting and In vitro Differentiation
  • 05:49Results: Representative In vitro Th Differentiation Data
  • 06:40Conclusion

Summary

Automatic Translation

Naïve CD4+ T cells polarize to various subsets depending on the environment at the time of activation. The differentiation of naïve CD4+ T cells to various effector subsets can be achieved in vitro through the addition of T cell receptor stimuli and specific cytokine signals.

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