An ELISA offering a novel quantitative approach is described. It specifically detects disease-associated α-synuclein (αSD) in a transgenic mouse model (M83) of synucleinopathy using several antibodies against either the Ser129 phosphorylated αS form or the C-terminal part of the protein.
Bétemps, D., Verchère, J., Mougenot, A., Lachmann, I., Morignat, E., Antier, E., Lakhdar, L., Legastelois, S., Baron, T. Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein. J. Vis. Exp. (99), e52752, doi:10.3791/52752 (2015).