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Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System
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Chapters
- 00:05Title
- 01:54Feeding 96-well Plate Stem Cell Colonies
- 03:26Passaging 96-well Plate Stem Cell Colonies
- 06:03Results: Representative Plate Based Robotic Stem Cell Culture
- 07:36Conclusion
The protocols described allow laboratories to perform scalable, adherent stem cell culture in high throughput with minimal labor, experience and equipment investment cost using a programmable liquid handling robot and 96-well plates. iPSCs passaged more than 20 times on this system maintained pluripotency, normal karyotypes and differentiated into cardiomyocytes.
