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Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration
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Developmental Biology
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JoVE Journal Developmental Biology
Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration

Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration

DOI:
10.3791/52756-v

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10:22 min

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June 25, 2015

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, ,
1Institute for Biochemistry and Molecular Biology,Ulm University

Chapters

  • 00:05Title
  • 01:51Generation of TetActivator, TetResponder Double Transgenic Fish Lines
  • 03:51Tail Fin Amputation and Induction of Transgene Expression in the Regenerating Fin
  • 05:11Characterization of TetResponder Expression in Fin Regenerates
  • 08:08Results: Analysis of Fin Regeneration Using the TetOn System
  • 09:44Conclusion

Summary

Automatic Translation

Automatic Translation

Here we outline the workflow for using the TetON system to achieve tissue-specific gene expression in the adult regenerating zebrafish tail fin.

Tags

Keywords: ZebrafishTetON SystemInducible Gene ExpressionTail Fin RegenerationMolecular MechanismsTransgenic LinesGene Expression Analysis

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