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Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination
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Immunology and Infection
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JoVE Journal Immunology and Infection
Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

DOI:
10.3791/52771-v

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11:07 min

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April 29, 2015

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1Department of Pharmaceutical Sciences,University of Michigan, 2Biointerfaces Institute,University of Michigan, 3Department of Biomedical Engineering,University of Michigan

Chapters

  • 00:05Title
  • 01:50Interbilayer-crosslinked Multilamellar Vesicle (ICMV) Synthesis and Characterization
  • 04:36Whole Animal Imaging of Antigen-specific, Luciferase-expressing CD8+ T Cell Expansion after Nanoparticle Vaccination
  • 06:47Peptide-MHC Tetramer Staining of PBMCs for Antigen-specific CD8+ T Cell Analysis
  • 08:27Results: ICMV Vaccination Elicits Endogenous OVA-specific CD8+ T Cell Expansion
  • 10:23Conclusion

Summary

Automatic Translation

Automatic Translation

We describe whole-animal imaging and flow cytometry-based techniques for monitoring expansion of antigen-specific CD8+ T cells in response to immunization with nanoparticles in a murine model of vaccination.

Tags

Keywords: Nanoparticle VaccineCD8+ T CellWhole-animal ImagingFlow CytometryAntigen-specific T Cell ResponseInterbilayer-crosslinked Multilamellar Vesicles (ICMVs)Bioluminescence ImagingTetramer Staining

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