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Biology

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HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins
 

HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

Article DOI: 10.3791/52772
June 10th, 2015

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Summary June 10th, 2015

Expression of malarial proteins in cell based systems remains challenging. We demonstrate two step and one step IVT (in vitro translation) cell free expression systems for expressing malarial recombinant rhoptry proteins from HeLa cells. We use a Ni-resin affinity based purification system to purify the rhoptry proteins.

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