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HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins
HeLa Based Cell Free Expression Systems for Expression of <em>Plasmodium</em> Rhoptry Proteins
JoVE Journal
Biology
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JoVE Journal Biology
HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

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09:03 min

June 10, 2015

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09:03 min
June 10, 2015

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Expression of malarial proteins in cell based systems remains challenging. We demonstrate two step and one step IVT (in vitro translation) cell free expression systems for expressing malarial recombinant rhoptry proteins from HeLa cells. We use a Ni-resin affinity based purification system to purify the rhoptry proteins.

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