Biology
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HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins
Chapters
Summary June 10th, 2015
Expression of malarial proteins in cell based systems remains challenging. We demonstrate two step and one step IVT (in vitro translation) cell free expression systems for expressing malarial recombinant rhoptry proteins from HeLa cells. We use a Ni-resin affinity based purification system to purify the rhoptry proteins.
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