Journal
/
Biology
/
HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

DOI:
10.3791/52772-v

•

09:03 min

•

June 10, 2015

•

,
1Department of Biological, Geological, and Environmental Sciences,Cleveland State University

Chapters

  • 00:05Title
  • 02:12Protein Expression Using In Vitro Human Cell Free Expression System
  • 03:21Purification of Expressed Recombinant Proteins
  • 05:05Western Blotting
  • 06:52Results: Purple Color Band on Nitrocellulose Paper During Developing Step Confirms the Successful Expression of Proteins
  • 08:11Conclusion

Summary

Automatic Translation

Automatic Translation

Expression of malarial proteins in cell based systems remains challenging. We demonstrate two step and one step IVT (in vitro translation) cell free expression systems for expressing malarial recombinant rhoptry proteins from HeLa cells. We use a Ni-resin affinity based purification system to purify the rhoptry proteins.

Tags

Keywords: HeLa Cell-free ExpressionPlasmodium ProteinsMalariaVaccine CandidatesCell-free Expression SystemsProtein ExpressionIn Vitro Human Cell-free ExpressionPF3D7 0114100PF3D7 0925900PF3D7 1361800Protein YieldAffinity Purification

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52772?article_type=v

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
check_url/52772?article_type=v

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
check_url/52772?article_type=v

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
check_url/52772?article_type=v

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
check_url/52772?article_type=v

Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles
check_url/52772?article_type=v

In vitro Reconstitution of the Active T. castaneum Telomerase
check_url/52772?article_type=v

Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer
check_url/52772?article_type=v

Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries
check_url/52772?article_type=v

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
check_url/52772?article_type=v

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Read Article