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Developmental Biology

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JoVE Journal Developmental Biology
Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
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Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Article DOI: 10.3791/52795 • 08:56 min • June 9th, 2015
June 9th, 2015 •
1,3, 2, 3, 3, 3, 3
1Research Center Growth and Signaling, INSERM U845, Institut Cochin, 2Unit for Biology of Lymphocyte Populations, Immunology Department, Institut Pasteur and CIMI, Unity of Treg Biology and Therapy, University of Pierre & Marie Curie, 3Unit for Lymphopoiesis, Immunology Department, INSERM U668, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur

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This article describes in vivo and in vitro methodology to characterize the thymic settling progenitors by the analysis of the kinetics of generation, phenotype and numbers of their T cell progeny.

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Thymic Settling Progenitors Mouse Embryo In Vivo Assay In Vitro Assay T Cell Development Lymphopenic Patients Hanging Drop Method CD45 Allotypic Markers Colonizing Irradiated Fetal Thymic Lobes Cell Autonomous Differences Biologic Properties Competitive Selective Pressures Grafted Thymic Lobes Immunodeficient Male Recipient Mice Mature T Cell Progeny Peripheral Immune System Colonization Of Tissues And Organs Fetal Thymic Organ Cultures CD3+ Cells Dendritic Epithelial T Cells Peripheral Blood Monitoring
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