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Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
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Developmental Biology
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JoVE Journal Developmental Biology
Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

DOI:
10.3791/52795-v

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08:56 min

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June 09, 2015

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, , , , ,
1Research Center Growth and Signaling, INSERM U845,Institut Cochin, 2Unit for Biology of Lymphocyte Populations, Immunology Department, Institut Pasteur and CIMI, Unity of Treg Biology and Therapy,University of Pierre & Marie Curie, 3Unit for Lymphopoiesis, Immunology Department, INSERM U668,University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur

Chapters

  • 00:05Title
  • 01:51Embryo Dissection
  • 02:52Thymus Isolation
  • 03:49E14 Thymic Lobe Progenitor Colonization: Hanging Drop Technique
  • 04:53Grafting Under the Kidney Capsule
  • 06:22Results: Representative Thymic Settling Progenitor (TSP) Propagation
  • 08:07Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes in vivo and in vitro methodology to characterize the thymic settling progenitors by the analysis of the kinetics of generation, phenotype and numbers of their T cell progeny.

Tags

Keywords: Thymic Settling ProgenitorsT Cell DevelopmentLymphopenic PatientsFetal Thymic Organ CultureCD45 Allotypic MarkersDendritic Epithelial T CellsPeripheral Immune SystemThymus GraftingCD3-/- Mice

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