Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

S&#233;bastien Meghezi; Dawit G. Seifu; Nina Bono; Larry Unsworth; Kibret Mequanint; Diego Mantovani

doi:10.3791/52812

Journal

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Bioengineering

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Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

20,765 Views

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09:23 min

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June 16, 2015

DOI:

10.3791/52812-v

DOI:

10.3791/52812-v

•

09:23 min

June 16, 2015

•

1 Views

Sébastien Meghezi¹, Dawit G. Seifu^1,2, Nina Bono^1,3, Larry Unsworth^2,4,5, Kibret Mequanint⁶, Diego Mantovani^1,2

¹Laboratory for Biomaterials and Bioengineering, Department Min-Met-Materials Eng & CHU de Québec Research Center, Canada Research Chair I for the Innovation in Surgery,Laval University, ²NSERC CREATE Program for Regenerative Medicine (NCPRM),Laval University, ³Department Electronics, Information and Bioengineering,Politecnico di Milano, ⁴Department of Chemical and Materials Engineering,University of Alberta, ⁵National Institute for Nanotechnology,National Research Council (Canada), ⁶Department of Chemical and Biochemical Engineering,University of Western Ontario

Summary

In this work, we present a technique for the rapid fabrication of living vascular tissues by direct culturing of collagen, smooth muscle cells and endothelial cells. In addition, a new protocol for the mechanical characterization of engineered vascular tissues is described.