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Immunology and Infection
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JoVE Journal Immunology and Infection
Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
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Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Article DOI: 10.3791/52833-v • 15:58 min • July 11th, 2015
July 11th, 2015 •
1,2, 1, 3, 3, 4, 1, 1
1Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 2Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, 3UK Veterinary Laboratories Agency, 4Visual Services, National Centers for Animal Health, Animal and Plant Health Inspection Service, United States Department of Agriculture

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Summary
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Long-term cultured interferon-γ enzyme-linked immunospot assay is used as a measure of central memory responses and correlates with protective anti-mycobacterial vaccine responses. With this assay, peripheral blood mononuclear cells are stimulated with mycobacterial antigens and interleukin-2 for 14 days, enabling differentiation and expansion of central memory T cells.

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Long-term Cultured Interferon-β Enzyme-linked Immunospot Assay Assessing Effector T Cell Responses Memory T Cell Responses Cattle Infection Control Contraction Phase Memory T Cells Lifetime Functionally Distinct Subsets Lymph Node Homing Receptors Central Memory T Cells C-C Chemokine Receptor 7 CD45RO Secondary Lymphoid Organs Effector Memory T Cells CCR7 Peripheral Or Inflamed Tissues Effector Cytokines Interferon-β Release Assays Bovine Tuberculosis Diagnosis Human Tuberculosis Diagnosis Effector Memory T Cell Responses CD4+ T Cells Vaccination Efficacy Prediction
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