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Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
This content is Free Access.
1Department of Organismic and Evolutionary Biology,Harvard University, 2Department of Immunology and Infectious Diseases,Harvard T.H. Chan School of Public Health, 3Faculty of Medicine and Pharmacy,Cheikh Anta Diop University, 4School of Nursing and Health Sciences,Simmons College, 5Institute of Infectious Diseases,Broad Institute
Chapters
- 00:05Title
- 01:58Template Preparation
- 03:46PCR Amplification
- 05:37HRM Analysis
- 08:26Results: Blocked-probe-based and MAAB Increases HRM Genotyping Sensitivity
- 09:46Conclusion
While high resolution melting analysis offers the ability to differentiate between single nucleotide polymorphisms in a heterogeneous population, mutant allele amplification bias can increase its ability to detect alleles present at relatively low percentages within a sample. This protocol describes improvements that improve the sensitivity of high resolution melting analysis.
