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JoVE Journal
Biology

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Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
 

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Article DOI: 10.3791/52840
August 16th, 2015

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Summary August 16th, 2015

This protocol describes the use of bromodeoxyuridine (BrdU) uptake to permit the temporal tracking of cells that were in S phase at a specific point in time. Addition of DNA dyes and antibody labeling facilitates detailed analysis of the fate of the S phase cells at later times.

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