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Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5
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Chapters
- 00:05Title
- 01:14Pre-Amplification of HCAdV Vectors in the Producer Cell Line 116
- 05:00Large Scale Amplification of High-capacity Adenovirus Vectors in 116 Cells in Suspension
- 08:49Purification of High-capacity Adenovirus
- 11:58Results: Characterization of the Final Virus Preparations
- 12:34Conclusion
A protocol for generation of high-capacity adenoviral vectors lacking all viral coding sequences is presented. Cloning of transgenes contained in the vector genome is based on homing endonucleases. Virus amplification in producer cells grown as adherent cells and in suspension relies on a helper virus providing viral genes in trans.
