Electrophysiological Motor Unit Number Estimation (MUNE) Measuring Compound Muscle Action Potential (CMAP) in Mouse Hindlimb Muscles

W. David Arnold; Kajri A. Sheth; Christopher G. Wier; John T. Kissel; Arthur H. Burghes; Stephen J. Kolb

doi:10.3791/52899

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Electrophysiological Motor Unit Number Estimation (MUNE) Measuring Compound Muscle Action Potential (CMAP) in Mouse Hindlimb Muscles

JoVE Journal
Behavior

This content is Free Access.

JoVE Journal Behavior

Electrophysiological Motor Unit Number Estimation (MUNE) Measuring Compound Muscle Action Potential (CMAP) in Mouse Hindlimb Muscles

21,023 Views

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09:07 min

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September 25, 2015

DOI:

10.3791/52899-v

DOI:

10.3791/52899-v

•

09:07 min

September 25, 2015

•

12 Views

W. David Arnold^1,2,3, Kajri A. Sheth¹, Christopher G. Wier⁴, John T. Kissel^1,3, Arthur H. Burghes^1,3,4, Stephen J. Kolb^1,3,4

¹Department of Neurology,The Ohio State University Wexner Medical Center, ²Department of Physical Medicine and Rehabilitation,The Ohio State University, ³Department of Neuroscience,The Ohio State University Wexner Medical Center, ⁴Department of Biochemistry and Pharmacology,The Ohio State University Wexner Medical Center

Summary

We present refined protocols that allow in vivo monitoring of motor unit function in the mouse. Techniques to measure compound muscle action potential (CMAP) and motor unit number estimation (MUNE) in the mouse hind limb muscles innervated by the sciatic nerve are described.