Protocols for Robust Herbicide Resistance Testing in Different Weed Species

Silvia Panozzo; Laura Scarabel; Alberto Collavo; Maurizio Sattin

doi:10.3791/52923

Protocols for Robust Herbicide Resistance Testing in Different Weed Species

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Protocols for Robust Herbicide Resistance Testing in Different Weed Species

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10:52 min

July 2, 2015

DOI: 10.3791/52923-v

Silvia Panozzo¹, Laura Scarabel¹, Alberto Collavo¹, Maurizio Sattin¹

¹Institute of Agro-environmental and Forest Biology (IBAF),National Research Council (CNR), Italy

A robust and flexible approach to confirm herbicide resistance in weed populations is presented. This protocol allows the herbicide resistance levels to be inferred and applied to a wide range of weed species and herbicides with minor adaptations.

The overall goal of this procedure is to test and confirm herbicide resistance in a wide range of weed species and herbicides, as well as to infer the herbicide resistance levels. This is accomplished by first breaking the dormancy of seeds collected from plants that had survived an herbicide treatment to obtain simultaneous germination and seedling emergence. A period of seed vernalization raging from a few days to more than a week is required.

The second step is to pour an auger medium with potassium nitrate into plastic dishes. The Vern seeds are then placed on the solidified auger and the dishes are transferred into a germination cabinet with the optimum conditions for each weed species. Next 15 to 20 seedlings are transplanted into plastic trays filled with a standard potting mix.

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