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Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
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Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Article DOI: 10.3791/52925
September 24th, 2015 •
1, 2
1Broad Institute, 2Department of Biological Engineering, Massachusetts Institute of Technology

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September 24th, 2015

We describe an endpoint digital assay for quantifying nucleic acids with a simplified (analog) readout. We measure bulk fluorescence of droplet-based digital assays using a standard qPCR machine rather than specialized instrumentation and confirm our results by microscopy.

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