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JoVE Journal
Biology

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qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping
 

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Article DOI: 10.3791/52941
May 25th, 2015

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Summary May 25th, 2015

Designer chromosomes of the Synthetic Yeast Genome project, Sc2.0, can be distinguished from their native counterparts using a PCR-based genotyping assay called PCRTagging, which has a presence/absence endpoint. Here we describe a high-throughput real time PCR detection method for PCRTag genotyping.

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