A Thrombotic Stroke Model Based On Transient Cerebral Hypoxia-ischemia

The overall goal of this procedure is to establish a simple thrombotic stroke model with a stable brain infarction and to evaluate the protective effects of tissue plasminogen, activator, or TPA therapy. First, the induction of transient cerebral hypoxia ischemia or THI is demonstrated via the double ligation of the right common carotid artery or RCCA and the exposure of the ligated animal to hypoxic conditions for the induction of thrombosis. After a 30 minute hypoxic condition, the two knots on the carotid artery are released.

In the final step, the administration and evaluation of the effects of TPA therapy are shown. Alternatively, realtime recording of the cerebral blood flow, the formation of the thrombosis and the degree of vessel obstruction can be investigated. This master can introduce thrombotic stroke in adult mice and rest using their own coagulation system.