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Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model
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Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model

Article DOI: 10.3791/53019-v • 09:31 min • October 2nd, 2015
October 2nd, 2015 •
1, 1, 2, 1
1Bill Walsh Translational Cancer Research Laboratory, Kolling Institute of Medical Research, Royal North Shore Hospital, University of Sydney, 2Northern Blood Research Laboratory, Kolling Institute of Medical Research, Royal North Shore Hospital, University of Sydney

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Summary
Automatic Translation

The generation of an orthotopic rat model of pleural malignant mesothelioma by implantation of II-45 mesothelioma cells into the pleural cavity of immune competent rats is presented. A flow cytometric method to analyze seven immune cell subsets in these animals from a 25 µl blood sample is also described.

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Orthotopic Implantation Peripheral Immune Cell Monitoring II-45 Syngeneic Rat Mesothelioma Model Immune-based Treatments For Cancer Vaccines Immune Checkpoint Inhibitors Tumor Microenvironment Pre-clinical Testing Orthotopic Models Pleural Malignant Mesothelioma Disease Progression Circulating Immune Cells Flow Cytometry Assay Lymphocytes Monocytes Neutrophils T-cell Subsets CD4 And CD8 B-cells Natural Killer Cells Neutrophil To Lymphocyte Ratio Monitoring Disease Progression Response To Immune-based Treatments
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