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Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model
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Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model

Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model

DOI:
10.3791/53019-v

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09:31 min

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October 02, 2015

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, , ,
1Bill Walsh Translational Cancer Research Laboratory, Kolling Institute of Medical Research,Royal North Shore Hospital, University of Sydney, 2Northern Blood Research Laboratory, Kolling Institute of Medical Research,Royal North Shore Hospital, University of Sydney

Chapters

  • 00:05Title
  • 01:44In Vivo Cell Implantation
  • 03:46Tail Vein Blood Collection
  • 05:04Bead-based Immune Cell Profiling and Analysis
  • 06:42Results: Representative Circulating Immune Cell Counts from Rats with Mesothelioma
  • 08:35Conclusion

Summary

Automatic Translation

Automatic Translation

The generation of an orthotopic rat model of pleural malignant mesothelioma by implantation of II-45 mesothelioma cells into the pleural cavity of immune competent rats is presented. A flow cytometric method to analyze seven immune cell subsets in these animals from a 25 µl blood sample is also described.

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Keywords: Orthotopic ImplantationPeripheral Immune Cell MonitoringSyngeneic Rat Mesothelioma ModelImmune-based TreatmentsTumor MicroenvironmentFlow CytometryLymphocytesMonocytesNeutrophilsT-cellsB-cellsNatural Killer CellsNeutrophil To Lymphocyte Ratio

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