Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles

The overall goal of the following experiment is to outline a step-by-step process of preparing and characterizing drug loaded my cells before evaluating them in disease states of interest. This is achieved by preparing the My cells by solvent casting method to generate reproducible my cells for the study. As a second step, my cell loading stability and size are assessed, which determines the ability of the my cells to load and retain clinically relevant concentrations.

Next, the rate of drug release from the My cells is determined in order to ensure my cells have appropriate in vitro release characteristics before assessing in vivo stability. The results show that reproducibly generated stable my cells can provide a platform to pursue drug delivery in various disease states based on the assessment techniques presented here. The main advantage of this technique or existing methods like equilibrium loading or dialysis methods, is that the myosins can be prepared much faster with reproducible drug loading and size.