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Enrichissement de matrice extracellulaire de protéines à partir de tissus et la digestion en peptides pour l'analyse de spectrométrie de masse
JoVE Journal
Biology
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JoVE Journal Biology
Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

Enrichissement de matrice extracellulaire de protéines à partir de tissus et la digestion en peptides pour l'analyse de spectrométrie de masse

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07:28 min

July 23, 2015

DOI:

07:28 min
July 23, 2015

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Summary

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This protocol describes a procedure for enriching ECM proteins from tissues or tumors and deglycosylating and digesting the ECM-enriched preparations into peptides to analyze their protein composition by mass spectrometry.

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