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A Screenable In Vivo Assay for Mitochondrial Modulators Using Transgenic Bioluminescent Caenorhabditis elegans
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A Screenable In Vivo Assay for Mitochondrial Modulators Using Transgenic Bioluminescent Caenorhabditis elegans

A Screenable In Vivo Assay for Mitochondrial Modulators Using Transgenic Bioluminescent Caenorhabditis elegans

DOI:
10.3791/53083-v

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11:43 min

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October 16, 2015

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, ,
1Institute of Medical Sciences,University of Aberdeen

Chapters

  • 00:05Title
  • 02:33Nematode Culture (Days 1-6)
  • 05:33Nematode Treatment (Day 7)
  • 07:44Nematode Fluorescence and Bioluminescence Quantification (Day 8)
  • 09:01Results: Representative C. elegans Bioluminescence after Drug Treatment
  • 10:50Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol is described for in vivo detection of effects of mitochondrial inhibitors in the model organism Caenorhabditis elegans and for identification of potential enhancing compounds. This protocol can be used to screen drug libraries for compounds modulating mitochondrial function.

Tags

Keywords: Caenorhabditis ElegansBioluminescentLuciferaseATP LevelsMitochondrial ModulatorsIn Vivo AssayScreeningParaquatRotenoneOxaloacetateFirefly Luciferase InhibitorsOxidative PhosphorylationDrug ToxicityMulticellular Organism

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