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Utilizzando cellule Caco-2 allo Studio Lipid Trasporto dall'intestino
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JoVE Journal Biology
Using Caco-2 Cells to Study Lipid Transport by the Intestine
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Utilizzando cellule Caco-2 allo Studio Lipid Trasporto dall'intestino

DOI:
10.3791/53086-v

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07:00 min

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August 20, 2015

•

,
1Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy,California Northstate University, 2Department of Biomedical Sciences, James H. Quillen College of Medicine,East Tennessee State University

Chapters

  • 00:05Title
  • 01:07Maintenance of Caco-2 Cells
  • 02:24Stimulating Lipoprotein Secretion and Lipoprotein Isolation
  • 04:35Transmission Electron Microscopy Analysis
  • 05:42Results: Analysis of Lipoproteins Secreted by Caco-2 Cells
  • 06:35Conclusion

Summary

Automatic Translation

Automatic Translation

Caco-2 cells can serve as an in vitro model to study the enterocyte transport of lipids, and lipid-soluble drugs/vitamins. The permeable membrane system separates the apical from the basolateral compartment, while the lentivirus expression system offers an effective gene overexpression method. The isolation of lipoproteins is confirmed by TEM.

Tags

Caco-2 CellsIntestinal Lipid TransportIn Vitro ModelDietary Fat AbsorptionLipid-soluble Drugs And VitaminsPermeable Membrane SystemPolarized CellsLentivirus Expression SystemIntestinal LipoproteinsTransmission Electron Microscopy

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