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JoVE Journal Bioengineering
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In Situ Mapping of the Mechanical Properties of Biofilms by Particle-tracking Microrheology

Article DOI: 10.3791/53093 • 12:58 min • December 4th, 2015
December 4th, 2015 •
1,2, 2,3,4,5, 2,3,4,6, 2,3
1Interdisciplinary Graduate School, Nanyang Technological University, 2Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, 3School of Biological Sciences, Nanyang Technological University, 4Centre for Marine Bio-Innovation, University of New South Wales, 5School of Biological, Earth and Environmental Sciences, University of New South Wales, 6School of Biotechnology and Biomolecular Sciences Sciences, University of New South Wales

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Summary
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Particle-tracking microrheology investigates the viscoelasticity of materials. Here, the technique is used to determine the viscoelasticity, creep compliance and effective crosslinking roles of different matrix components of a bacterial biofilm. The matrix consists of polymeric substances secreted by the bacteria and its components determine biofilm structure and mechanical properties.

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In Situ Mapping Mechanical Properties Biofilms Particle-tracking Microrheology Bacterial Cells Surface-attached Biofilm Communities Extracellular Polymeric Substances (EPS) Physical Scaffold Proteins Exopolysaccharides DNA EPS Composition Mechanic Properties Biofilm Structures Streamers Environmental Cues Quantitative Descriptions EPS Components Rheology Flow Of Matter Shear Stress Spreading Of Biofilm Particle-tracking Microrheology Viscoelasticity Effective Crosslinking Roles Matrix Components Biofilm Development Micro-scale Measurement
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