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JoVE Journal
Developmental Biology

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Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila
 

Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila

Article DOI: 10.3791/53149
September 30th, 2015

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Summary September 30th, 2015

Mitochondria play central roles in the regulation of metabolism and homeostasis. Subtle changes in mitochondrial metabolism that affect organismal physiology could be difficult to detect in whole organism metabolomics studies. Here we describe an isolation method that enhances the detection of subtle metabolic shifts in Drosophila melanogaster.

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