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Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
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1Department of Biomedical Science,The Bateson Centre, University of Sheffield, 2Institute of Genetic Medicine,Newcastle University, 3Department of Cardiovascular Science,The Bateson Centre, University of Sheffield, 4School of Biochemistry,University of Bristol, 5Biology Department,University of York
Chapters
- 00:05Title
- 01:09Microinjecting Xenopus Embryos
- 02:10Excising Animal Cap Explants
- 04:17Animal Explant Imaging
- 06:04Results: Diffusion Analysis of Fluorescently Tagged Ligands in Xenopus Animal Cap Explants
- 07:38Conclusion
The manuscript here provides a simple set of methods for analysing the secretion and diffusion of fluorescently tagged ligands in Xenopus. This provides a context for testing the ability of other proteins to modify ligand distribution and allowing experiments that may give insight into mechanisms regulating morphogen gradients.
