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Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments
Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments

Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments

9,453 Views

09:40 min

September 30, 2015

DOI:

09:40 min
September 30, 2015

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Summary

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This protocol describes a method to dissect, experimentally manipulate and culture whole retinal explants from chicken embryos. The explant cultures are useful when high success rate, efficacy and reproducibility are needed to test the effects of plasmids for electroporation and/or reagent substances, i.e., enzymatic inhibitors.

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