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Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments
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Developmental Biology
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JoVE Journal Developmental Biology
Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments

Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments

DOI:
10.3791/53202-v

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09:40 min

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September 30, 2015

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, ,
1Department of Neuroscience, Biomedical Center,Uppsala University

Chapters

  • 00:05Title
  • 01:33Egg Handling and Eye Collection
  • 03:21Preparation of Whole Retinal Explants
  • 04:15Electroporation of Whole Retinal Explants
  • 07:28Results: Uses of Cultured Whole Retinal Explants
  • 08:59Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a method to dissect, experimentally manipulate and culture whole retinal explants from chicken embryos. The explant cultures are useful when high success rate, efficacy and reproducibility are needed to test the effects of plasmids for electroporation and/or reagent substances, i.e., enzymatic inhibitors.

Tags

Keywords: Whole Retinal ExplantsChicken EmbryosElectroporationChemical ReagentsExperimental ModelCentral Nervous SystemIntraocular InjectionsEx Vivo CultureControlled ExposureRapidReproducibleCost-effective

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