Journal
/
Medicine
/
VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma

VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma

DOI:
10.3791/53215-v

•

15:07 min

•

December 28, 2015

•

, , , , ,
1Department of Medicine,Weill Cornell Medical College, 2Institute for Computational Biomedicine,Weill Cornell Medical College, 3Department of Pathology and Laboratory Medicine,Weill Cornell Medical College

Chapters

  • 00:05Title
  • 00:41DNA Extraction from Tumor Samples
  • 04:00DNA Quality Assessment
  • 05:33VDJ PCR
  • 07:30VDJ Amplicon Library Preparation
  • 12:32Results: VDJ-sequencing Traces Clonal Evolution Patterns of B Cell Lymphoma Relapse
  • 14:27Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes an approach to interrogate the recombined immunoglobulin heavy chain VDJ regions of lymphomas by deep-sequencing and retrieve VDJ rearrangement and somatic hypermutation status to delineate clonal architecture of individual tumor. Comparing clonal architecture between paired diagnosis and relapse samples reveals lymphoma relapse clonal evolution modes.

Tags

Keywords: VDJ-seqImmunoglobulin Heavy ChainB Cell LymphomaClonal EvolutionNext-generation SequencingSomatic HypermutationClonal HeterogeneityTumor RelapseMinimal Residual DiseaseImmune Repertoire

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/53215?article_type=v

Dissection of Human Vitreous Body Elements for Proteomic Analysis
check_url/53215?article_type=v

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice
check_url/53215?article_type=v

Production and Detection of Reactive Oxygen Species (ROS) in Cancers
check_url/53215?article_type=v

DNA Vector-based RNA Interference to Study Gene Function in Cancer
check_url/53215?article_type=v

Deep Neuromuscular Blockade Leads to a Larger Intraabdominal Volume During Laparoscopy
check_url/53215?article_type=v

Identification of Disease-related Spatial Covariance Patterns using Neuroimaging Data
check_url/53215?article_type=v

Detection of Architectural Distortion in Prior Mammograms via Analysis of Oriented Patterns
check_url/53215?article_type=v

Gene-environment Interaction Models to Unmask Susceptibility Mechanisms in Parkinson’s Disease
check_url/53215?article_type=v

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene
check_url/53215?article_type=v

A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting

Read Article