Journal
/
Developmental Biology
/
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements

Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements

DOI:
10.3791/53216-v

•

10:39 min

•

October 30, 2015

•

, , , , ,
1The Department of Human Nutrition, Foods, and Exercise,Virginia Tech, 2The Metabolic Phenotyping Core,Virginia Tech, 3Seahorse Bioscience

Chapters

  • 00:05Title
  • 01:30Setup
  • 02:53Running the Assay
  • 07:45Results: Microplate Based Assay Measures Oxygen Consumption in Skeletal Muscle Mitochodria
  • 10:05Conclusion

Summary

Automatic Translation

Automatic Translation

The methods presented provide step-by-step instructions for the performance of a collection of microplate based respirometric assays using isolated mitochondria from minimal quantities of mouse skeletal muscle. These assays are able to measure mechanistic changes/adaptations in mitochondrial oxygen consumption in a commonly used animal model.

Tags

Key Words: Mitochondrial RespirationSkeletal MuscleMouseMicroplateHigh-throughputIsolated MitochondriaRespiratory Measurements

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/53216?article_type=v

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
check_url/53216?article_type=v

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
check_url/53216?article_type=v

Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients
check_url/53216?article_type=v

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection
check_url/53216?article_type=v

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
check_url/53216?article_type=v

Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles
check_url/53216?article_type=v

Application of Chronic Stimulation to Study Contractile Activity-induced Rat Skeletal Muscle Phenotypic Adaptations
check_url/53216?article_type=v

Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
check_url/53216?article_type=v

X-ray Diffraction of Intact Murine Skeletal Muscle as a Tool for Studying the Structural Basis of Muscle Disease
check_url/53216?article_type=v

Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure

Read Article