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From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
 

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

Article doi: 10.3791/53245
July 4th, 2016

Summary July 4th, 2016

β-barrel outer membrane proteins (OMPs) serve many functions within the outer membranes of Gram-negative bacteria, mitochondria, and chloroplasts. Here, we hope to alleviate a known bottleneck in structural studies by presenting protocols for the production of β-barrel OMPs in sufficient quantities for structure determination by X-ray crystallography or NMR spectroscopy.

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