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A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

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08:34 min

October 16, 2015

DOI:

08:34 min
October 16, 2015

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Summary

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A protocol for small molecular drug screening based on in-situ synthesis of ultrasmall fluorescent gold nanoclusters (Au NCs) using drug-loaded protein as template is presented. This method is simple to determine the binding affinity of drugs to a target protein by a visible fluorescent signal emitted from the protein-templated Au NCs.

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