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JoVE Journal
Bioengineering

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Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
 

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Article DOI: 10.3791/53271-v 09:43 min August 10th, 2015
August 10th, 2015

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Summary

This protocol describes decellularization of Sprague Dawley rat kidneys by antegrade perfusion of detergents through the vasculature, producing acellular renal extracellular matrices that serve as templates for repopulation with human renal epithelial cells. Recellularization and use of the resazurin perfusion assay to monitor growth is performed within specially-designed perfusion bioreactors.

Tags

Epithelial Cell Repopulation Rodent Extracellular Matrix Scaffolds Renal Tissue Development Renal Scaffold Generation Decellularization Perfusion Bioreactor Vessel Seeding Renal Cortical Tubular Epithelial Cells ECM Niche
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