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Bioluminescence Imaging of an Immunocompetent Animal Model for Glioblastoma
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Medicine
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JoVE Journal Medicine
Bioluminescence Imaging of an Immunocompetent Animal Model for Glioblastoma

Bioluminescence Imaging of an Immunocompetent Animal Model for Glioblastoma

DOI:
10.3791/53287-v

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09:17 min

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January 15, 2016

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, , ,
1Department of Neurological Surgery,University of California San Francisco, 2Department of Neurological Surgery,Northwestern University Feinberg School of Medicine, 3Biochemistry and Molecular Genetics,Northwestern University Feinberg School of Medicine

Chapters

  • 00:05Title
  • 01:00Induction of GL261 Firefly Luciferase Reporter Expression
  • 02:31In Vitro Proliferation Assay
  • 03:40Tumor Cell Implantation
  • 06:16Bioluminescence Imaging of GL261 Tumor Growth
  • 07:26Results: Representative In Vitro and In Vivo GL261 Luciferase Expression
  • 08:31Conclusion

Summary

Automatic Translation

Automatic Translation

GL261 glioma cells provide a useful immunocompetent animal model of glioblastoma. The goals of this protocol are to demonstrate proper techniques for monitoring intracranial tumor growth using in vivo bioluminescence imaging, and to verify the utility of luciferase-modified GL261 cells for studying tumor immunology and immunotherapeutic approaches for treating glioblastoma.

Tags

Keywords: Bioluminescence ImagingImmunocompetent Animal ModelGlioblastomaGL261 Murine Tumor CellsLuciferase ModificationTumor Cell ProliferationIn Vivo MonitoringTumor GrowthImmunotherapyIn Vitro Proliferation AssayMTS ReagentAbsorbance Measurement

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