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Immunology and Infection

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JoVE Journal Immunology and Infection
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Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

Article DOI: 10.3791/53296-v • 10:22 min • November 12th, 2015
November 12th, 2015 •
1,2, 1
1Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, 2Instituto de Biotecnologìa, Universidad Nacional Autónoma de México

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Summary
Automatic Translation

We provide a novel strategy to isolate viral replication compartments (RC) from adenovirus (Ad)-infected human cells. This approach represents a cell-free system that can help to elucidate the molecular mechanisms regulating viral genome replication and expression as well as regulation of viral-host interactions established at the RC.

Tags

Isolation Viral Replication Compartment-enriched Sub-nuclear Fractions Adenovirus Normal Human Cells Infection Host Cell Nucleus Nuclear Microenvironments Replication Compartments Viral-induced Nuclear Domains Viral Genome Localization Replication Transcription Post-transcriptional Processing Cellular Proteins Antiviral Response Tumor Suppressor Proteins DNA Damage Response (DDR) Components Innate Immune Response Factors Composition Associated Activities Adenoviral Replication Compartments DNA Viruses Cell Nucleus
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