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JoVE Journal
Biology
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Isolation and Compositional Analysis of Plant Cuticle Lipid Polyester Monomers
 

Isolation and Compositional Analysis of Plant Cuticle Lipid Polyester Monomers

Article DOI: 10.3791/53386
November 22nd, 2015

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Summary November 22nd, 2015

Lipid polyesters constitute the structural components of two cell wall modifications, the plant cuticle and suberin-containing diffusion barriers. In this video, we describe a method to depolymerize cutin from whole delipidated leaves. The method can be applied to investigating mutants compromised in either cutin or suberin biosynthesis.

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