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Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
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Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

DOI:
10.3791/53422-v

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12:36 min

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January 14, 2016

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, , ,
1Centro de Biotecnología y Genómica de Plantas (CBGP),Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)-Universidad Politécnica de Madrid

Chapters

  • 00:05Title
  • 01:11Crosslinking of Plant Material
  • 02:53Preparation of Antibodies
  • 04:26Chromatin Extraction
  • 07:25Immunoprecipitation of the Protein of Interest and Rescue of DNA
  • 09:45Results: Chromatin Immunoprecipitation Assay to Assess EBS-DNA Interactions in Arabidopsis
  • 11:13Conclusion

Summary

Automatic Translation

Automatic Translation

Chromatin immunoprecipitation is a powerful technique for the identification of DNA binding sites of Arabidopsis proteins in vivo. This procedure includes chromatin cross-linking and fragmentation, immunoprecipitation with selective antibodies against the protein of interest, and qPCR analysis of bound DNA. We describe a simple ChIP assay optimized for Arabidopsis plants.

Tags

Keywords: Chromatin ImmunoprecipitationArabidopsisProtein-DNA InteractionsCross-linkingTranscription FactorsChromatin OrganizationPlant BiologyMagnetic BeadsChIP Dilution Buffer

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