Imaging Subcellular Structures in the Living Zebrafish Embryo

Peter Engerer; Gabriela Plucinska; Rachel Thong; Laura Trov&ograve;; Dominik Paquet; Leanne Godinho

doi:10.3791/53456

Journal

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Developmental Biology

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Imaging Subcellular Structures in the Living Zebrafish Embryo

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Imaging Subcellular Structures in the Living Zebrafish Embryo

11,618 Views

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11:19 min

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April 02, 2016

DOI:

10.3791/53456-v

DOI:

10.3791/53456-v

•

11:19 min

April 02, 2016

•

11606 Views

Peter Engerer¹, Gabriela Plucinska^1,2, Rachel Thong^1,3, Laura Trovò¹, Dominik Paquet^4,5,6, Leanne Godinho¹

¹Institute of Neuronal Cell Biology,Technische Universität München ²Cell Biology, Department of Biology, Faculty of Science,Utrecht University ³Faculty of Biology,Ludwig-Maximilians-Universität-München ⁴Adolf-Butenandt-Institute, Biochemistry,Ludwig-Maximilians-Universität-München ⁵German Center for Neurodegenerative Diseases ⁶Laboratory of Brain Development and Repair,The Rockefeller University

Summary

Imaging the dynamic behavior of organelles and other subcellular structures in vivo can shed light on their function in physiological and disease conditions. Here, we present methods for genetically tagging two organelles, centrosomes and mitochondria, and imaging their dynamics in living zebrafish embryos using wide-field and confocal microscopy.