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JoVE Journal
Biology

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Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate
 

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Article doi: 10.3791/53464
December 12th, 2015

Summary December 12th, 2015

Glycolysis is a defining metabolic marker in multiple biological systems. Monitoring glycolysis by measuring the extracellular flux of H+ is common, but requires correction to be quantitative and unambiguous. Here, we demonstrate how to gather and correct extracellular flux data to distinguish between respiratory and glycolytic sources of extracellular acidification.

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