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Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate
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JoVE Journal Biology
Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

DOI:
10.3791/53464-v

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06:47 min

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December 12, 2015

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,
1Touro University California College of Pharmacy, 2Buck Institute for Research on Aging

Chapters

  • 00:05Title
  • 00:38Measuring Buffering Power with an Extracellular Flux Instrument
  • 02:30Measuring Buffering Power with an External pH Meter
  • 03:43Extracellular Flux Assay
  • 04:48Results: Contributions of Glycolysis and Respiration to the Proton Production Rate (PRR) after Correction
  • 05:49Conclusion

Summary

Automatic Translation

Automatic Translation

Glycolysis is a defining metabolic marker in multiple biological systems. Monitoring glycolysis by measuring the extracellular flux of H+ is common, but requires correction to be quantitative and unambiguous. Here, we demonstrate how to gather and correct extracellular flux data to distinguish between respiratory and glycolytic sources of extracellular acidification.

Tags

Extracellular Acid ProductionGlycolytic RateRespirationMitochondrial Oxygen ConsumptionLactateCO2C2C12 MyoblastsExtracellular Acidification

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