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JoVE Journal
Biology

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DNA Electroporation, Isolation and Imaging of Myofibers
 

DNA Electroporation, Isolation and Imaging of Myofibers

Article DOI: 10.3791/53551
December 23rd, 2015

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Summary December 23rd, 2015

This protocol utilizes electroporation to introduce and express fluorescently labeled proteins in mouse muscle fibers. Following recovery after electroporation, fibers are isolated. Individual fibers are then imaged using high resolution confocal microscopy to visualize muscle structure.

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