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JoVE Journal Developmental Biology
Generazione di primaria fibroblasti culture da orecchio e coda tessuti di topo
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Generazione di primaria fibroblasti culture da orecchio e coda tessuti di topo

Article DOI: 10.3791/53565-v • 06:35 min • January 10th, 2016
January 10th, 2016 •
1, 1,2
1Immunology Program, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 2NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore

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Descriviamo una procedura sperimentale semplice e rapido per la generazione di fibroblasti primari dalle orecchie e le code di topi. La procedura non richiede addestramento speciale animali e può essere utilizzato per la generazione di colture di fibroblasti da spighe conservati a temperatura ambiente per 10 giorni.

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Biologia dello Sviluppo Numero 107 Estrazione fibroblasti cellule primarie mouse code orecchie
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