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Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation
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Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

English

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21,417 Views

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09:30 min

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March 04, 2016

DOI:

10.3791/53579-v

DOI:
10.3791/53579-v

•

09:30 min
March 04, 2016

•

3 Views
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1Burns Centre and Emilia Romagna Regional Skin Bank, 2Human Brain Wave srl, 3Plastic and Reconstructive Surgery,AOU “Ospedali Riuniti”, 4Department of Public Health, Experimental Medicine, Anatomy and Forensic,University of Pavia, 5C.H.T Centre for Health Technologies,University of Pavia

Summary

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The protocol describes a new method to disaggregate human tissues and to create autologous micro-grafts that, combined with collagen sponges, give rise to human bio-complexes ready to use in the treatment of skin lesions. Further, this system preserves cell viability of micro-grafts at different times after mechanical disaggregation.

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Cite this Article

Purpura, V., Bondioli, E., Graziano, A., Trovato, L., Melandri, D., Ghetti, M., Marchesini, A., Cusella De Angelis, M. G., Benedetti, L., Ceccarelli, G., Riccio, M. Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation. J. Vis. Exp. (109), e53579, doi:10.3791/53579 (2016).

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